tumorigenic human cell lines  (ATCC)

Journal: PLOS Pathogens

Article Title: Tripartite motif-containing 34 (TRIM34) protein interacts with the nucleocytoplasmic transport machinery and negatively modulates antiviral responses

doi: 10.1371/journal.ppat.1014142

Figure Lengend Snippet: (A, B) Human 293T cells were transfected with the plasmid pCAGGs-TRIM34-FLAG or the empty control plasmid. (A) At 24h post-transfection, cells were non-treated or treated with type I IFN. At 24h post-IFN-treatment, CXCL10, IFNB1, IFNL1 and IFIT2 expression was measured by RT-qPCR and mRNA levels were expressed as fold-change (increases) in comparison to mock-treated cells transfected with the empty plasmid, used as control. (B) At 24h post-IFN-treatment, cells were infected with rVSV-GFP (MOI 0.1) and viral titers were measured by lysis plaque assay at 24hpi. Data represents means and SDs of results from triplicate wells. Three different experiments were performed, with similar results. *p < 0.05 for comparisons between empty and TRIM34 overexpressed cells using Student’s t-test with Holm-Šídák correction . Human 293T cells (C, F) , human lung adenocarcinoma-derived cells A549 cells (D, G), or the non-tumorigenic human bronchial epithelial BEAS-2B (E, H) were transfected twice with NT control siRNA or TRIM34 siRNA, 24h apart, for two consecutive days. On day 3, cells were treated with IFN for an additional 24h. (C, D, E) TRIM34, and (F, G, H) CXCL10, IFNB1, IFNL1 and IFIT2 expression was measured by RT-qPCR and mRNA levels were expressed as fold-change (increases) in comparison to mock-treated cells, transfected with the NT siRNA, used as control. (A-H) Data represents means and SDs of results from triplicate wells. Three different experiments were performed, with similar results. *p < 0.05 for comparisons between control and TRIM34 knocked-down cells using Student’s t-test with Holm-Šídák correction .

Article Snippet: Non-tumorigenic human bronchial epithelial cell line BEAS-2B (ATCC CRL-9690), was obtained from Carlos López-Larrea (Health Research Institute of the Principality of Asturias (ISPA), Spain).

Techniques: Transfection, Plasmid Preparation, Control, Expressing, Quantitative RT-PCR, Comparison, Infection, Lysis, Plaque Assay, Derivative Assay

Journal: PLOS Pathogens

Article Title: Tripartite motif-containing 34 (TRIM34) protein interacts with the nucleocytoplasmic transport machinery and negatively modulates antiviral responses

doi: 10.1371/journal.ppat.1014142

Figure Lengend Snippet: (A) Human 293T cells were transfected with the pCAGGS plasmids encoding TRIM34-FLAG or the empty plasmid, as control. At 24h post-transfection, cells were mock-infected or infected with IAV or IAV-ΔNS1. At 24hpi, the levels of CXCL10, IFNB1, IFNL1, and IFIT2 mRNAs were evaluated by RT-qPCR, and expressed as fold-change (increases) in comparison to mock-infected cells, transfected with the empty plasmid, used as control. (B) Human 293T cells were transfected twice with the NT control siRNA or TRIM34 siRNA every 24h for two consecutive days. On day 3, cells were infected with IAV or IAV-ΔNS1 (MOI 1) for an additional 24h. The levels of CXCL10, IFNB1, IFNL1 and IFIT2 were measured by RT-qPCR and mRNA levels were expressed as fold- change (increases) in comparison to mock-infected cells transfected with the NT siRNA, used as controls. Human A549 (C) or BEAS-2B (D) cells were transfected twice with the NT control siRNA or TRIM34 siRNA every 24h for two consecutive days. On day 3, cells were infected with IAV for an additional 24h. (B, C, D) The levels of CXCL10, IFNB1, IFNL1 and IFIT2 were measured by RT-qPCR and mRNA levels were expressed as fold change (increases) in comparison to mock-infected cells transfected with the NT siRNA, used as controls. (A-D) Data represents means and SDs of results from triplicate wells. Three different experiments were performed, with similar results. *p < 0.05 for comparisons between empty and TRIM34 overexpressed cells, or NT siRNA and TRIM34 knocked-down cells, using Student’s t-test with Holm-Šídák correction. (E, F) A549 cells were infected with the viruses rIAV-Venus, rIAV-mCherry and rIAV-TRIM34 at MOI 1, and the levels of (E) IAV NP-vRNA, and (F) CXCL10, CCL2, IFNL1 and IFIT2 mRNAs were determined at 24hpi. (E, F) Data represents means and SDs of results from triplicate wells. Three different experiments were performed, with similar results. *p < 0.05, **p < 0.01, ***p < 0.001 for comparisons between rIAV-TRIM34 with rIAV-Venus and rIAV-mCherry using one-way ANOVA followed by Dunnett’s post-hoc test.

Article Snippet: Non-tumorigenic human bronchial epithelial cell line BEAS-2B (ATCC CRL-9690), was obtained from Carlos López-Larrea (Health Research Institute of the Principality of Asturias (ISPA), Spain).

Techniques: Transfection, Plasmid Preparation, Control, Infection, Quantitative RT-PCR, Comparison

Journal: Frontiers in Pharmacology

Article Title: Selective biological activity of parthenolide derivatives — stizolin, stizolicin, and izospiciformin — isolated from the leaves of Stizolophus balsamita , in different breast cancer molecular subtypes in vitro

doi: 10.3389/fphar.2026.1799963

Figure Lengend Snippet: Assessment of Stizolophus balsamita extract, stizolin, stizolicin, and izospiciformin on breast cancer cells’ viability. MCF7, MDA-MB-231, SK-BR-3, and MCF-12A cells were treated for 24 (A) , 48 (B) , or 72 (C) h with a wide range of concentrations of studied compounds, i.e., 1–100 µg/mL. The mean ± SD from three experiments is shown. Statistically significant differences versus control samples are shown: *p < 0.05; p < 0.005; *p < 0.001.

Article Snippet: The human breast cancer cell lines: MCF7 (ER+, PR+, HER2-), MDA-MB-231 (ER-, PR-, HER2-), SK-BR-3 (ER-, PR-, HER2+), and MCF-12A human non-tumorigenic breast cell line (ER+, PR+, HER2-) were obtained from the American Type Culture Collection (ATCC, HTB-22, HTB-26, HTB-30, CRL-10782, respectively).

Techniques: Control